GenMute™ siRNA Transfection Reagent for Primary Keratinocytes

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单价: 1980.00
品牌: SignaGen
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更新: 2021-06-01
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货号: SL100568-KER
供应商: 美中清水湾生物科技有限公司
数量: 现货 大量
英文名: GenMute™ siRNA Transfection Reagent for Primary Keratinocytes
保质期: 1年
保存条件: 4°C
规格: 毫升

Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on primary keratinocytes. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute
 Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 


Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS
~70%
40 nM

1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
90%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf
温馨提示:不可用于临床治疗。