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份 库存999份
| 货号： | B600274-0500 |
| 供应商： | 生工生物工程（上海）股份有限公司 |
| 数量： | 现货 |
| 英文名： | AccB1I |
| 规格： | 500 U |
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Recognition sites G↑GYRCC
Source Acinetobacter calcoaceticus B1
Assayed on Lambda DNA
Optimal buffer K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.) + BSA
Optimal temperature 37°C
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Ligations After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methlation effect Methylation sensitivity not tested
Thermal inactivation enzyme is inactivated by incubation at 65°C for 20 mins.
Campatible Ends BanI, BshNI, BspT107I, HgiCI
温馨提示：不可用于临床治疗。
[VIP第1年] 指数:1
