sGAG. Alcian blue-binding assay_其它cDNA相关_cDNA及合成纯化_生化试剂库_商城

货号：	GAG 201
供应商：	Euro Diagnostica
英文名：	sGAG. Alcian blue-binding assay
保质期：	1年
保存条件：	-80
规格：	kit

The most abundant heteropolysaccharides in the body are the glycosaminoglycans (GAGs). They are located primarily on the surface of cells or in the extracellular matrix. As an example, cartilage is composed to a large extent of GAGs, which are the dominant part of the proteoglycan Aggrecan.

GAGs are negatively charged long unbranched polymeric polysaccharides composed of repeating units of disaccharides containing one uronic acid or galactose and one amino sugar, either N-acetyl-glucosamine or N-acetylgalactosamine. The variation in charge may be very large since each disaccharide is more or less sulphated.

Based on GAG’s high negative charge, a number of dye-binding procedures for their measurement have been developed. In most cases, however, they are not applicable to biological material without different forms of pre-treatment such as protease digestion. The present assay makes use of the dye Alcian blue (C.I.# 74240) which has a long history as histological tissue staining reagent. Alcian blue is a tetravalent cation with a hydrophobic core. The four charges allow the dye to bind to negatively charged polymers such as GAGs at high ionic strength, in contrast to other cationic dyes which are all monovalent. The molecular structure of Alcian blue, i.e. the plane tetragonal hydrophobic core with positive charges at its corners, may facilitate formation of aggregates of several molecules side by side rather than micelle formation. The ionic strength, pH and presence of detergents will affect the size of these aggregates in solution.

The ionic bonding between cationic dyes (such as Alcian blue) and the negatively charged GAGs are generally thought to be proportional to the number of negative charges present on the GAG chain, i.e. both sulphate and carboxyl groups.

Product code：GAG 201 RUO
Tests：200
Units：ug/mL
Calibrators：6
Range：0-400 ug/mL
Incubation time：7x15 min
Detection system：600-620 nm (preferably 620 nm)
Availability：Research use only

温馨提示：不可用于临床治疗。

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sGAG. Alcian blue-binding assay

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