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| 品牌： | Invitrogen |
| 货号： | V800-20 |
| 数量： | 大量 |
| 供应商： | 天津子涵生物科技有限公司 |
| 规格： | 100 TESTS |
and designed for high-level expression, purification, and detection of
recombinant proteins in mammalian hosts. High-level stable and non-replicative
transient expression can be carried out in most mammalian cells. The vectors
contain the following elements:
Three reading frames to facilitate in frame cloning with a C-terminal tag
encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide
Human cytomegalovirus immediate-early (CMV) promoter for high-level
expression in a wide range of mammalian cells
Episomal replication in cell lines that are latently infected with SV40 or that
express the SV40 large T antigen (e.g., COS7)
The control plasmid, pcDNA™3.1/myc-His/lacZ, is the pcDNA™3.1/myc-His C
vector with a 3.2-kb fragment containing the -galactosidase gene cloned in frame
with the C-terminal peptide (see page 9). It is included for use as a positive
control for transfection, expression, purification, and detection in the cell line of
choice.
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