货号：	K-3300
保存条件：	4°C, and -20°
规格：	kit

PI(3)PMass ELISA Kit
96-well ELISA Assay for Detection and Quantification of PI(3)P from cells
Product Number: K-3300

Catalog No.: K-3300

Description: The PI(3)P Mass ELISA measures the amount of PI(3)P extracted from cells by means of a competitive ELISA.

Assay Range: 1,600 to 1.56 pmol PI(3)P
Sample Type: Dried down lipid extracted from cells
Sample Volume: 180 μL/sample, run in triplicate.

The PI(3)P Mass ELISA Kit allows for the determination of PI3-K activity by quantifying the amount of PI(3)P found in cells. After following a non-radioactive lipid extraction protocol, the PI(3)P samples obtained from millions of cells are detected on a competitive ELISA. The cellular PI(3)P samples are mixed and incubated with a PI(3)P Detector protein, then added to a PI(3)P-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the PI(3)P protein binding to the plate. The assay is sensitive to 1.5 pmol PI(3)P. The colorimetric signal is inversely proportional to the amount of PI(3)P obtained.

Format:

96-well Competitive ELISA

Storage:

2-8°C, and -20°C

Related Products:

PIP Mass Assays
K-3000, Class III PI3K Elisa Kit
Antibody:Anti-hVps34 Antibody for IP(Z-R015), Anti-hVps34 Antibody for WB(Z-R016)

Reference:

1. Nonradioactive Quantification of Phosphoinositide 3-kinase (PI3K) Products from Breast Cancer Cell Lines
2. Quantification of PI3-Kinase Products from Breast Cancer Cell Lines

温馨提示：不可用于临床治疗。