澳洲胎牛血清/ES tested FBS

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单价: 6800.00
品牌: Biosun
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更新: 2021-06-02
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货号: FB-1001S
供应商: Biosera
数量: 2000
英文名: ES tested FBS
保质期: 5年(-25度冻存)
保存条件: -25度冻存
规格: 500ml

胎牛血清生产过程在一个可控的条件下进行。SIP灭菌、无菌过滤和分装作为关键的灭菌过程证实是有效的。产品生产符合质量管理标准ISO9001。此种血清出厂前做过胚胎干细胞培养、定向诱导分化检测,常用来培养各种干细胞、原代细胞、神经细胞等。
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Embryonic stem (ES) cells tested FBS

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst. The stem cells can be maintained in vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst.  ES cells may differentiate in vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors.  General culture conditions are well established and usually require ES cells to be grown on an inactive feeder cell layer or with Leukemia Inhibitory Factor (LIF) in culture.
When growing ES cells, one of the most important parameters is the maintenance of the cells in the undifferentiated state.  Pre-screening of the serum is essential before using it for the culture of ES cells.  Various lots of sera (10% Foetal Bovine Serum ) are screened by the plating efficiency method.  Plating efficiency measures the number of undifferentiated cell colonies (ES-D3 murine pluripotent cells, ATCC, CRL-1934) formed under given conditions.  The results are used as an indication of the quality of sera for the growth and maintenance of undifferentiated stem cells.
温馨提示:不可用于临床治疗。