Sigma Eagle 培养基,含Earle's盐、L-谷氨酰胺和NaHCO3 M4655

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单价: 303.00
品牌: Sigma
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更新: 2021-07-26
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货号: M4655
供应商: 杭州乐乾科学仪器有限公司
数量: 258
规格: M4655-500ML/M4655-1L
M4655
MSDS
低限量 Eagle 培养基 
With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture (Sigma)
 
货号与规格 价格 (CNY)

M4655-500ML

343.98

M4655-1L

537.03

M4655-6X500ML

1,755.00

M4655-6X1L

2,737.80

M4655-24X500ML

6,177.60


产品描述

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’ or Earle’s salts has broadened the usefulness of this medium.

性质
Related Categories Apoptosis and Cell Cycle, Cell Culture Media & Reagents, Classic Media, Classical Media, Core Bioreagents,
Culture, Drug Discovery, General Stem Cell Biology, Life Science Reagents for Cell Culture, Media, Minimum Essential Medium Eagle (MEM), Other Apoptosis Related Reagents, Research Essentials, Stem Cell Differentiation, Stem Cell Expansion, 干细胞生物学, 细胞信号转导和神经科学, 细胞培养, 细胞生物学, 经典培养基和盐
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Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   NaHCO3: yes
Earle’s salts (5% CO2): yes
HEPES: no
L-glutamine: yes
phenol red: yes
sodium pyruvate: no
storage temp.   2-8°C

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