sigma西格玛低限量 Eagle 培养基M8042

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单价: 465.00
品牌: Sigma-Aldrich
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更新: 2021-05-29
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货号: M8042
数量: 2368
保存条件: 2-8℃
规格: M8042-500ML
M8042
MSDS
低限量 Eagle 培养基 
Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture (Sigma)
 
货号与规格 价格 (CNY)

M8042-500ML

527.67

M8042-6X500ML

2,679.30

M8042-24X500ML

9,231.30


产品描述

General description

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. See formulation for details. Often used in cytogenetic analysis when supplemented with FBS.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.292 g/L L-glutamine

Application

Minimum Essential Medium Eagle is used for primary osteoblast cultures[3]and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[2]

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[1]

性质

Related Categories Classical Media, Culture, General Stem Cell Biology,Minimum Essential Medium Eagle (MEM), Stem Cell Differentiation,
Stem Cell Expansion, 干细胞生物学, 细胞培养, 细胞生物学, 经典培养基和盐
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Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   NaHCO3: yes
L-glutamine: no
phenol red: yes
HEPES: no
sodium pyruvate: yes
storage temp.   2-8°C
温馨提示:不可用于临床治疗。