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Application• For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Direct NAD/NADH Assays in 96-Well PlateNEW!!!Key Features• Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Method• F530/585nmSamples• Cell, tissue extracts etc. (Direct NAD/NADH Assays in 96-Well PlateNEW!!!)Species• AllProcedure• 10 minSize• 100 testsDetection Limit• 0.02 μMShelf Life• 6 monthsMore Details• Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. BioAssay Systems EnzyFluo™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
产品订货信息：
EFND-100 NAD/NADH检测试剂盒（荧光） 100次检测 ￥3492
EFND-048 NAD/NADH检测试剂盒（荧光） 48次检测 ￥2247
