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分类名称 :血液尿液化学,代谢产物分析产品介绍 : 测定方法： 比色法（565nm）定量测定D-乳酸的含量。 产品说明： 乳酸是在缺氧或厌氧条件下由乳酸脱氢酶(LDH)催化生成。因此乳酸浓度的监测是组织内氧需求与利用平衡的良好指标。同时对细胞和动物生理学研究也很重要。D-乳酸仅少量存在于动物体内并且对动物样品中D-乳酸的测量常作为评定细菌感染与否。简单，直接和自动化的乳酸浓度检测方法是非常可取的。博世生物技术提供的D-乳酸测试盒是基于乳酸脱氢酶催化氧化乳酸，其中形成的NADH耦合到甲臜(MTT)发色体上。产物的颜色强度(565nm)与样品中乳酸的浓度成正比。 产品特点： 敏感、准确: 96-孔板检测范围为0.05～2 mM乳酸，对于含有酚红的细胞培养物样品，检测范围为0.1 ～1 mM 乳酸。 储存条件： 所有试剂均于-20°C 保存。 DESCRIPTION Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for Dlactatein animal samples is a means to determine the presence of bacterial infection. KEY FEATURES Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1mM D-lactate in 96-well plate assay. Storage conditions Store all reagents at -20°C. Shelf life: 6 months after receipt. 编号： EDLC-048 D-乳酸检测试剂盒 48次检测 ￥2150 EDLC-100 D-乳酸检测试剂盒 100次检测 ￥3378
