Catalog Number:
500-P270Bt
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Anti-Human Maspin specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen:
E.coli derived Recombinant Human Maspin (PeproTech catalog #130-12)
Application:
ELISA
DirectTo detect hMaspin by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMaspin. 
ELISA: Direct
SandwichTo detect hMaspin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Maspin (500-P270) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMaspin. Western Blot
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western Transfer (Western Blot)
Research Interests:
| Angiogenesis/ Cardiovascular | | Apoptosis |
| Cancer | | Wound Healing |
Country of Origin:
USA
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[VIP第1年] 指数:1
