试剂盒Section 1 Protein Analysis and ModificationSensitive assays of maleimide and thiol groups are required for the efficient conjugation of proteins that areexpensive and available only in small amounts. A variety of crosslinking reagents with a maleimide group arewidely used for crosslinking proteins to proteins or proteins to other biomolecules. There are few reagents orassay kits available for quantifying the number of maleimide groups that are introduced into the first protein.
Protein tools | Cat# |
Maleimide quantitation | SG5523 SG5525 |
Thiol quantitation | SG5524 |
Amplite™ Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*
Cat # | Size | Price | Storage |
SG5523 | 1 kit(200 assays) | ¥2,028 | <-15℃ |
Broad Application: uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. Can be used for quantifying maleimide group in a variety of molecules such as proteins. Sensitive: Detect as low as 10 picomoles of maleimide. Ex/Em : 490 nm/520 nm Amplite™ Fluorimetric Thiol Quantitation Assay Kit *Green Fluorescence*
Cat # | Size | Price | Storage |
SG5524 | 1 kit(200 assays) | ¥2,028 | <-15℃ |
Broad Application: Can be used for quantifying thiol and sulfide in a variety ofbiological systems (e.g., plasma, urine and cell extracts)Sensitive: Detect as low as 1 picomole of thiol.Ex/Em : 490 nm/520 nm Amplite™ Colorimetric Maleimide Quantitation Kit
Cat # | Size | Price | Storage |
SG5525 | 1 kit (100 assays) | ¥2,028 | <-15℃ |
Broad Application: Provides a rapid and robust colorimetric method for quantifying maleimide groups. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. Sensitive: lower than Cat SG5523 常用荧光生物学染料荧光探针是一个标记在生物样本上或是在刺激下发生反应的荧光基团。荧光探针以高灵敏度和高选择性,应用于检测复杂生物分子(如活体细胞)的特殊组分。活性荧光染料被广泛用于修饰氨基酸,多肽,蛋白质(尤其是抗体),寡核苷酸,核酸,碳水化合物和其他生物分子。 在活性荧光染料中,由于胺基含量丰富或容易被引入到生物分子,与胺基反应的染料形成的偶合物常用于免疫组织化学,组织化学,荧光原位杂交(FISH),细胞示踪,受体结合和其他生物学应用。与巯基反应的荧光染料开发成的探针常应用于蛋白质的结构和功能研究。此外,一些含胺的荧光试剂也可用于修饰生物分子,尤其是标记糖蛋白。 本公司推出高质量的荧光染料,荧光的稳定性和持续性可以媲美甚至优于国内外的同类产品,广泛应用于蛋白(FAM 系列、TF系列、i F系列)和核酸标记(TQ系列、TF系列),细胞标记(7-ADD,DAPI)和示踪(CFDA、VFSE),凋亡检测(Apopxin系列),细胞膜电位(JC-1、JC-10)和钙离子通道检测(Fura系列、Indo系列、QF系列、QR系列等)。
蛋白、多肽和核酸标记 | |
SG114 | 5-FAM, SE |
SG117 | 6-FAM, SE |
SG121 | FITC |
SG363 | 5-TAMRA |
SG373 | 5-TAMRA, SE |
SG376 | 6-TAMRA, SE |
SG392 | 6-ROX, SE |
SG480 | Sulforhodamine 101 sulfonyl chloride |
SG2203 | Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å* |
SG2204 | Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å* |
SG2223 | Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å* |
SG2224 | Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å* |
SG2226 | Tide Quencher™ 3 maleimide [TQ3 maleimide] |
SG2228 | Tide Quencher™ 3 phosphoramidite [TQ3 maleimide] |
SG2230 | Tide Quencher™ 3 succinimidyl ester [TQ3 SE] |
SG2245 | Tide Fluor™ 2 acid [TF2 acid] |
SG2248 | Tide Fluor™ 2, succinimidyl ester [TF2 SE] |
SG2251 | Tide Fluor™ 2 phosphoramidite [TF2 CEP] |
SG2268 | Tide Fluor™ 3 acid [TF3 acid] *Superior replacement to Cy3* |
SG2269 | Tide Fluor™ 3 amine [TF3 amine] |
SG2270 | Tide Fluor™ 3 maleimide [TF3 maleimide] |
SG2271 | Tide Fluor™ 3 succinimidyl ester [TF3 SE] |
SG2278 | Tide Fluor™ 5 acid [TF5 acid] *Superior replacement to Cy5* |
SG2279 | Tide Fluor™ 5 amine [TF5 amine] |
SG2280 | Tide Fluor™ 5 maleimide [TF5 maleimide] |
SG2281 | Tide Fluor™ 5 succinimidyl ester [TF5 SE] |
SG2284 | Tide Fluor™ 5 phosphoramidite [TF5 CEP] |
SG2330 | Tide Fluor™ 7 acid [TF7 acid] *Superior replacement to Cy7* |
SG2331 | Tide Fluor™ 7 amine [TF7 amine] |
SG2332 | Tide Fluor™ 7 maleimide [TF7 maleimide] |
SG2333 | Tide Fluor™ 7, succinimidyl ester [TF7 SE] |
SG4505 | Sulfo-SMCC |
SG6021 | 6-TET phosphoramidite |
SG6024 | 6-HEX phosphoramidite |
SG17004 | AA-dUTP |
SG17006 | mFluor™ Violet 450-dUTP |
SG17007 | TF2-dUTP |
SG17008 | TF3-dUTP |
SG17010 | TF5-dUTP |
细胞标记 | |
SG17501 | 7-AAD [7-Aminoactinomycin D] |
SG17507 | DAPI |
SG17509 | DAPI Dilactate |
SG17520 | Hoechst 33258 |
SG17530 | Hoechst 33342 |
SG22002 | Calcein, AM |
SG22022 | CFDA, SE |
SG22023 | VFSE™ 450 |
SG22024 | VFSE™ 500 |
钙离子检测 | |
SG21020 | Fura-2, AM |
SG21030 | Indo-1, AM |
SG21080 | Quest Fluo-8™ |
SG21096 | Quest Fluo-8L™, |
SG21102 | Quest Fluo-8FF™ |
SG21116 | Fluo-2 AM |
SG21120 | Quest Rhod-4™, AM |
线粒体膜电位检测 | |
SG22200 | JC-1 |
SG22204 | JC-10 *Superior alternative to JC-1* |
酶底物 | |
SG11000 | Amplite™ ADHP |
SG14001 | FDG [Fluorescein di-β-D-galactopyranoside] |
细胞凋亡 | |
SG20070 | |