Anti-DARS2抗体

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单价: 1380.00
品牌: Ybscience
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更新: 2024-03-28
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 Anti-DARS2抗体

产品编号 YB-14198R
英文名称 DARS2
中文名称 天门冬氨酰tRNA连接酶2抗体
别    名 Aspartate tRNA ligase 2 mitochondrial; Aspartate tRNA ligase; Aspartate tRNA ligase mitochondrial; Aspartyl tRNA synthetase 2 (mitochondrial); Aspartyl tRNA synthetase mitochondrial; AspRS; FLJ10514; SYDM_HUMAN; LBSL; MT ASPRS; RP3 383J4.2.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 细胞生物  信号转导  新陈代谢  线粒体  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量 68kDa
细胞定位 细胞浆 线粒体
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DARS2:551-645/645 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009] Function: Aspartyl-tRNA synthetase (DARS) charges its cognate tRNA with aspartate during protein biosynthesis. Mammalian synthetases differ from those of prokaryotes and lower eukaryotes in that they associate as multienzyme complexes. Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. SWISS: Q6PI48 Gene ID: 55157 Database links:

Entrez Gene: 55157 Human

Entrez Gene: 538772 Cow

Entrez Gene: 226539 Mouse

Entrez Gene: 304919 Rat

GenBank: NM_018122.3 Human

Omim: 610956 Human

SwissProt: A6QPU5 Cow

SwissProt: Q6PI48 Human

SwissProt: Q8BIP0 Mouse

SwissProt: Q3KRD0 Rat

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.   
产品图片
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;  Incubation: Anti-DARS2 Polyclonal Antibody, Unconjugated(bs-14198R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining