预优化DNA转染试剂-C6 Glioma

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更新: 2021-05-21
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Description

GenJet™ DNA In Vitro Tranfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6 glioma cells. The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers. Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral transfection methods. The pre-optimized transfection reagent simplifies the transfection of C6 cells. Transfection efficiencies of over 60% can be easily achieved with excellent cell viabilities of above 90%.

Refer to the following optimal transfection conditions for maximal transfection efficiency on C6 glioma cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency  Transfection Results: Reporter Gene Plasmid Efficiency (GFP %)

~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter)  60%

Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet.pdf