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人Tie2 / Angpt信号定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis. Brief examples of how included genes may be grouped according to function are shown below:
- Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1
- Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA
- Motility: PLD1, MAP2K1, DOK2, NCK1, CRK
- Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1
- Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | ADSS | CDC42 | DOK2 | FOXO3 | HIF1A | ITGB5 | MMP2 | PDIA3 | PTPN11 | SELE | TEK | β-actin |
| B | AKT1 | CDKN1A | DOK4 | FYN | HRAS | KLF2 | MTOR | PGK1 | PXN | SELP | TIAM1 | GAPDH |
| C | AKT2 | CRK | EGR1 | GJA5 | ICAM1 | MAP2K1 | NCK1 | PIK3CA | RAC1 | SOS1 | TIE1 | LDHA |
| D | ALDOA | CTNNB1 | ELF2 | GRB14 | ILK | MAPK1 | NFKB1 | PIK3R1 | RAF1 | SPHK1 | TLN1 | NONO |
| E | ANGPT1 | CXCL12 | ELK1 | GRB2 | ITGA5 | MAPK14 | NOS3 | PIK3R2 | RASA1 | STAT1 | TNIP2 | PPIH |
| F | ANGPT2 | DIABLO | EPAS1 | GRB7 | ITGAV | MAPK3 | NOX1 | PLCG1 | RELA | STAT3 | TXNRD1 | GDC |
| G | ARHGAP5 | DIAPH1 | FES | GYS1 | ITGB1 | MAPK7 | NR4A1 | PLD1 | RHOA | STAT5A | VCAM1 | PPC |
| H | BIRC5 | DLL4 | FOXO1 | HAGH | ITGB3 | MEF2A | PAK1 | PTK2 | RIPK1 | STAT5B | VEGFA | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK029 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TEK |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TEK is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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