磷酸化丝氨酸单克隆抗体

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单价: 1580.00
品牌: HZbscience
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更新: 2020-09-15
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 Mouse Anti-Phosphoserine antibody

 

产品编号 hz-33200M
英文名称 Phosphoserine
中文名称 ***酸化丝氨酸单克隆抗体
别    名 phospho-Ser; phospho Ser; pS; p-S; pSER; p-SER; p-serine.  
   
产品类型 药物与化合物抗体 
研究领域 细胞生物  表观遗传学  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 7C11
交叉反应 Mouse, Phosphoserine
产品应用 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Phosphoserine: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background: Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.  SWISS: N/A CAS: 407-41-0 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.   
产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phosphoserine) Polyclonal Antibody, Unconjugated (bsm-33200M-7C11) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.