Minute TM 总蛋白提取试剂盒

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单价: 290.00
品牌: Inventbiotech
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更新: 2021-05-07
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货号: SD-001/SN-002-4T
保存条件: RT
规格: 4tests

MinuteTM 动物细胞/组织总蛋白提取试剂盒

 

目录号:SD-001/SN-002

 描述  

       动物细胞/组织总蛋白提取试剂盒,由优化细胞裂解液和0.6ml离心管柱组成,能够迅速从动物细胞和组织(无脊椎动物和脊椎动物)中提取总蛋白,用于蛋白质分析和进一步纯化。试剂盒同时提供天然和变性两种不同的裂解液,可供用户选择。离心管柱技术提取蛋白质,样品量最小可处理20ul,最大可达500ul。从细胞/组织中提取总蛋白质仅需1-8分钟,得率可达2-8mg/ml。提取的蛋白质样品可以应用于蛋白质分析和小规模蛋白层析纯化。

应用  

本试剂盒可以快速从无脊椎动物和脊椎动物培养的细胞和组织中分提取总蛋白,可应用于SDS-PAGE, immunoblottings, ELISA, IP,酶活性检测等其他应用

 

无蛋白丢失验证

 

 






操作步骤







 


 

SD-001/SN-002

操作流程图

观看操作视频点击

http://v.qq.com/x/page/t0328m0fj09.html



 

 Protocol  

请点击产品资料下载

产品订购信息  
Minute TM Total Protein   Extraction Kit  for Animal Cultured   Cells/Tissues (50 Tests) SD-001/SN-002 2875.00
Minute TM Total Protein   Extraction Kit  for Animal Cultured   Cells/Tissues (4 Tests) SD-001/SN-002 290.00
参考文献  

1. Wang M-J et al (2014). Reversal of hepatocyte senescence after continuous in vitro cell proliferation. Hepatology 60:349-361.

2. Xiang D. et al. (2014). Non-viral FoxM1 gene delivery to hepatocytes enhances liver repopulation. Cell Death and Disease 5, e1252; doi:10.1038/cddis.2014.230.

3. Ishikawa H. et al. (2014) L-Carnitine Prevents Progression of Non-Alcoholic Steatohepatitis in a Mouse Model with Upregulation of Mitochondrial Pathway. PLoS ONE 9(7): e100627. doi:10.1371/journal.pone.0100627.

4. Kaya M.K. et al. (2015) Effects of lapatinib and trastuzumab on vascular endothelial growth factor in experimental corneal neovascularization1. Clinical and Experimental Opthalmology. doi: 10.1111/ceo.12500.

5. M. Cuchra,et al. (2015). The role of base excision repair in the development of primary open angle glaucoma in the Polish population, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

(2015),http://dx.doi.org/10.1016/j.mrfmmm.2015.05.003

6.James B. Nardi.et al.(2016)Segmental pairs of giant insect cellsdischarge presumptive immune proteins at each larval molt. DevelopmentalBiology.DOI: http://dx.doi.org/10.1016/j.ydbio.2016.03.029

7.Cho S-M et al (2016).  Comparisonof hemocytic carbonic anhydrase activity of bivalves.Korean J. Malacol.32(1): 63-65.

8. Morimoto, K. et al. (2016). Intestinalbile acid composition modulates prohormone convertase 1β (PC1/3) expression andconsequent GLP-1 production in male mice. Endocrinology, 157(3),1071-1081.10.1210/en.2015-1551

9. Santosh K. Misra, et al (2016). Pro-haloacetateNanoparticles for Efficient Cancer Therapy via Pyruvate Dehydrogenase Kinase Modulation.Scientific Reports | 6:28196 | DOI: 10.1038/srep28196.

10. LI ZHANG et al2016. CHKAmediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator. ONCOLOGYLETTERS. DOI: 10.3892/ol.2016.4810

11. Shuji Hamauchi.et al(2016). Cellular Functionsand Gene and Protein Expression Profiles in Endothelial Cells Derived fromMoyamoya Disease-Specific iPS Cells. PLOS ONE|DOI:10.1371/journal.pone.0163561

12.K.M.I.Bashir.et al(2016). The Effect of Kanamycin and Tetracycline on Growthand Photosynthetic Activity of Two Chlorophyte Algae. BioMed ResearchInternational Volume 2016, Article ID 5656304, 8 pageshttp://dx.doi.org/10.1155/2016/5656304

13.K.Takahashi, et. al., Exercise combined with low-level GABAA receptor 

inhibitionup-regulates the expression of neurotrophins in the motor cortex, Neurosci. Lett.(2016),http://dx.doi.org/10.1016/j.neulet.2016.10.052.

温馨提示:不可用于临床治疗。