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份 库存999份
| 货号： | K600-100 |
| 保存条件： | -20度 |
| 供应商： | 北京博迈斯 |
| 英文名： | Sphingomyelin Quantification Colorimetric Assay Kit |
| 规格： | 100 assays |
Assay Kit Name:
Sphingomyelin Quantification Colorimetric Assay Kit
Kit Summary:
• Detection method - Absorbance (OD 570 nm)
• Species reactivity - N/A
• Applications:
-Sphingomyelin quantification in various biological samples
- Analysis of cell signaling pathway
Sample Type:
• Serum, plasma
• Tissue samples such as brain etc.
• Adhesion or suspension cells
Features & Benefits:
• Simple, rapid & convenient
• The assay can detect sphingomylein less than 1 µM in a variety of samples
Kit Components:
• SM Assay Buffer
• OxiRed Probe™
• Sphingomyelinase (Lyophilized)
• ALP Enzyme (Lyophilized)
• SM Enzyme Mix (Lyophilized)
• SM Standard (5 mM)
Description:
Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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